Variation in Chemical Composition of the Nervous System. As by G. B. Ansell

By G. B. Ansell

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1958) J. biol. C h e m . 2 3 3 , 1053. MOSER H . W . and KARNOVSKY M. L . (1959) J. biol. C h e m . 2 3 4 , 1990. Abstracts 45 REGIONAL P A T T E R N O F LDH-ISOENZYMES DURING POSTNATAL D E V E L O P M E N T OF THE RABBIT RETINA e . G . H o n e g g e r and W. M e i e r - R u g e N e u r o l o g i c a l Clinic and P a t h o l o g i c a l Institute of the U n i v e r s i t y of B a s e l , S w i t z e r l a n d . A method h a s been d e v e l o p e d (HONEGGER, 1964) for s e p a r a t i n g L D H - i s o e n z y m e s d i r e c t l y f r o m s m a l l t i s s u e s e c t i o n s .

Lipid phosphorus ( L - P ) and c h o l e s t e r o l v a l u e s i n c r e a s e d in both g r a y and white m a t t e r o v e r this p e r i o d , a s r e p o r t e d by o t h e r a u t h o r s . In the white m a t t e r of the new b o m , total fatty a c i d s (TFA) w e r e about 25%, p l a s m a l o g e n s at 10% of the adult c o n c e n t r a t i o n , and n e a r l y a l l fatty a c i d s w e r e e s t e r - l i n k e d . T F A doubled and p l a s m a l ­ o g e n s quadrupled during the f i r s t 8 months of l i f e .

H o n e g g e r and W. M e i e r - R u g e N e u r o l o g i c a l Clinic and P a t h o l o g i c a l Institute of the U n i v e r s i t y of B a s e l , S w i t z e r l a n d . A method h a s been d e v e l o p e d (HONEGGER, 1964) for s e p a r a t i n g L D H - i s o e n z y m e s d i r e c t l y f r o m s m a l l t i s s u e s e c t i o n s . With t h i s technique a h i s t o t o p o c h e m i c a l study w a s c a r r i e d out in o r d e r t o r e v e a l the c h a n g e s in the LDH i s o e n z y m e pattern during postnatal development of the rabbit retina.

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