By Professor Dr. Y. P. S. Bajaj (eds.)
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George infected with grapevine fanleaf virus (Fig. 8). In vitro techniques are also valuable for the detection of grapevine viruses. ) Fig. 8. Effect of 2-deoxy-D-glucose, an inhibitor of glycosylation, on grapevine fanleaf virus (GFLV) content of shoot tip cultures of fanleafinfected V. rupestris Scheele. Proliferating shoot tip cultures were grown for 2 weeks in media containing various amounts of the drug. They were then homogenized in buffer and subjected to enzyme-linked immunosorbent assay (ELISA) for GFLV detection (Monette 1985).
1983) and Grenan (1984) reported the existence of a morphogenic gradient along the shoots of the micropropagated vines, the base producing juvenile growth and the tip producing adult growth. If the tip of the micropropagated vine was not removed by pruning, the varietal leaf shape returned. The varietal leaf shape was then retained in cuttings taken from terminal portions of the vine. These authors postulated that the pruning (to two nodes) normally done each year left only buds which had progressed only slightly along the morphogenic gradient and that this resulted in an artificial perpetuation from year to year of juvenile characteristics.
1985) Embryo recovery and establishment of seedlings Improvement of seedless cvs. Spiegel-Roy et al. (1985) Dual culture with Plasmopara viticola Influence of CO2 on morphogenesis in vitro Screening of cvs. for resistance to downy mildew Accelerated return of 'adult' characteristics in micropropagated vines Barlass et al. ) 15 quality and with the same varietal characteristics as the mother plant. Grapevine micropropagation has been carried out by axillary shoot development on shoot tips (defined as the apical meristem plus a few subjacent leaf primordia) and by adventitious shoot production on either fragmented shoot apices or leaf blade explants (Thble 3).